Analysis of Opiates in Oral Fluids Utilizing LC-MS/MS

نویسندگان

  • Gregory Newland
  • Song Ye
چکیده

LC/MS/MS is a very selective and sensitive analytical technique for detection and quantification of analytes in complex matrices. As a result, the use of LC/MS/MS in forensic toxicological applications has been significantly increasing over the past decade. The use of oral fluid as an alternative biological matrix for drug testing has also increased. However, the sample volume is usually much smaller and drugs are present at lower concentrations in oral fluid when compared to urine or blood. The selectivity and sensitivity of LC-MS/MS make it ideally suited for analysis of oral fluid for the presence of opiates. A method for extraction, detection, and quantification of several opiates in oral fluid has been developed and discussed in this application note. The developed method demonstrates that the LLOQs are better than 0.5 ng/mL for all analytes, and the linear dynamic range is about three orders of magnitude. The ion ratios are reproducible throughout the entire dynamic range. LC-MS/MS has proven to be a powerful tool for oral fluid opiates sample confirmation and quantitation at low concentration levels.

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تاریخ انتشار 2011